One of the proven facts of genetics is induced mutagenesis the most successful, safe and low cost approach for producing the different varieties of a crop species. In the present scenario linseed (Linum usitatissimum L.) a medicinally as well as economically important plant of the family Linaceae, was selected for the present study for the enhancement of genetic variability by using induced mutagenesis technique. Healthy seeds of two linseed varieties were treated with different doses of hydrazine hydrate (HZ) for a short duration of six hours. The findings recorded were correlated with qualitative and quantitative traits to assess mutagenic potency and to produce desirable genetic variation in selected crop plant. The bio-physiological study showed a steady reduction in the germination percentage with the increasing doses of mutagens. The total chlorophyll content showed a significant negative shift from control to all treatment in both the varieties. The doses of hydrazine hydrate (0.2% and 0.3%) were found to be the most potent for the induction of the genetic variability in both the varieties. The lower and moderate doses were found to be applicable for the inducing the mutation in the agro-economical traits of linseed. In quantitative characters, lower biological destruction was evaluated with a high percentage of variance.