Male infertility, which corresponds to half of the cases in a couple infertility, is assessed primarily by sperm parameters such as sperm motility and vitality. These, according to WHO standards, provide information on the fertility potential of a man. Thus, patients with abnormal sperm parameters are more likely to fail in medically assisted procreation because a lack of sperm mobility and vitality reveals aberrations in the paternal genome. Phytotherapy, which highlights numerous molecules known for their therapeutic and anti-infectious, antioxidant, antibacterial, antifungal and other properties, seems to be suitable for improving spermatic parameters. Also, Oreganum vulgare, an essential oil that has already proven itself in traditional medicine and known for various curative actions, would be an asset in spermiology? Thus, in our study, we evaluated the effect of a supplementation of this essential oil in fresh sperm ejaculates. The vitality and mobility of spermatozoa were the parameters studied after 24, 48 and 72 hours of suspension of a clean fraction of spermatozoa in Origanum vulgare.