India has different biotic and abiotic stresses which initiate the decrease in the wheat production every year and due to this reason it is not accessible to majority of the population. Triticum aestivum (wheat) is known for its nutrient value and is grown on large scale in our country. It is declared as the healthiest diet compared to any other source. To improve the crop production of the wheat, Kinetin has been used by many researchers. Kinetin is a plant growth hormone isolated earlier by the scientists to promote the cell division and to encourage comparable growth. So, a low cost experiment was done in the plant tissue culture lab to cultivate the seeds in-vitro. These seeds were then treated with the various concentration of kinetin (0mg/l, 50mg/l, 100mg/l, and 150mg/l) and kept in the plant growth chamber to evaluate the expansion of seeds. These seeds were kept under observation for 7 days and then sub-cultured with MS- media representing that kinetinimproves crop salt tolerance; improvise heavy metal stress, and a plant phytohormone that further activates antioxidant defense system. Present study supports in-vitro growth of Triticumaestivumwith additional improved physical conditions. Cost effective and top quality seeds can be obtained in minimum time through this technique.