The study of heavy metals (Cd, Cr, and Ni) effect on biochemical parameters in Spinacea oleracea L. (Palak). Spinach (Spinacea oleracea L.) is an edible leafy vegetable plant in the family Amaranthaceae, spinach is an annual plant (sometimes biennial), Spinach shows the growth up to 30cm in tall. It can survive over winter and in temperate regions also. The leaves Spinach are alternate, ovate to triangular, and mostly variable in size that is about 2–12 cm long and 1–10 cm in broad, the larger leaves on the base of the plant and small leaves on the higher stem. Common spinach, S. oleracea, considered as long in the family Chenopodiaceae, but in 2003, that was merged into the family Amaranthaceae in the order Caryophyllales, spinach with raw contains 91% water, 4% carbohydrates, 3% protein, and it also contains negligible fat. Spinach is rich in carotenoids, body can convert it into vitamin-A. The present research study was conducted to know the toxicity nature of heavy metals in Spinacea oleracea L. leaf and its remediation. Pot culture experiments were conducted with three treatments till productivity levels at Greenhouse of Botanical Garden, Department of Botany, Osmania University, Hyderabad. The three treatments consist of Treatment I control without any addition of heavy metals to the soil, Treatment II heavy metals spiked into the soil and Treatment. III, 1 % of calcium hydroxide added along with heavy metals to the soil. The results showed when compared to treatment I and III the high concentrations of heavy metals (Ni, Cd and Cr) are found in leaf of Spinacea oleracea L. in (Treatment II). In addition, the plants grown in treatment III with 1% Calcium hydroxide treated soil, reversed the growth suppression and inhibited the heavy metal toxicity in plants as evidenced by reduced heavy metal concentration in leaf. The study concludes that leafy vegetable Spinacea oleracea L. affected with heavy metals can be treated by using calcium hydroxide.