Abstract:

This study shows the effect of various parts of Cannabis sativa as a plant on some multidrug-resistant clinical isolates. Cannabis sativa plants and the ready-to-smoke popularly called skunk (SK) were obtained from the Nigeria Drug Law Enforcement Agency (NDLEA), Ado-Ekiti Unit, Ekiti State, Nigeria, and processed for laboratory analysis and standard microbiological assays. This study shows that Cannabis sativa’s potential and antimicrobial activity are valuable in treating multi antibiotics clinically resistant microorganisms. It was observed that n-haxane extracts of Cannabis sativa seed -A1, Cannabis sativa stem -A3, Cannabis sativaSkurk -A5 as well as chloroform extracts of extract Cannabis sativa seed -D1 and Cannabis sativa leaves-D2 are inhibitive against test organisms. The most active was extract A1 which showed a bactericidal effect against some enteric organisms such as Escherichia coli, Salmonella typhi and Salmonella pullorum tested. Others are Staphylococcus aureus, Bacillus cereus as well as bacteriostatic effect against Proteus mirabilis and Pseudomonas aeruginosa. Extract A3 showed bactericidal effect against Bacillus cereus and bacteriostatic effect against Proteus mirabilis. Similarly, Extract A5 and Extract D1 showed a bactericidal effect against Bacillus cereus. In comparison, Extract D2 and Extract E3 showed bactericidal effects against Salmonella typhi and Proteus mirabilis, respectively. The observation in this study shows that Extract A1 is highly effective against test organisms such as Salmonella pullorum and Bacillus cereus, where its bactericidal effect is greater than that of the control (Ofloxacin) used. It further suggests that Extracts A3, A5, D1, and D2 can be further studied by increasing the concentration and changing the solvent used for extraction.