Comparative study of two SARS-COV 2 qRT-PCR kits with two types of internal controlsx
Achraf Zakaria , Modou Mamoune Mbaye* , Noha Kobbi , Abdelaziz Soukri , Bouchra El Khalfi , Noureddine Louanjli
DOI : 10.10.46890/SL.2021.v02i06001
Abstract
Coronavirus is a virus that was unknown before the outbreak in Wuhan (China) in December 2019 but today it has become a major public health problem. Indeed, Covid-19 is an infectious and acute disease but it can also be very deadly especially with certain comorbidities such as hypertension, diabetes, coronary heart disease, cerebral infarction or even chronic bronchitis. The qRT-PCR is the reference method for the diagnosis of Covid-19. Nevertheless, it is a delicate technique whose result depends on several parameters such as the quality of the sample, the RNA extraction steps, the cDNA synthesis, the choice of the amplification kit, the choice of controls, the analysis and the validation of the results. Any anomalies in these steps may hinder the obtaining of relevant and reliable biological results. The objective of this paper is to verify the importance and analytical efficiency of the endogenous and exogenous controls used in two diagnostic tests for Sars-cov2 by qRT-PCR commonly used in the laboratory of medical analysis and biology of reproduction, Labomac Casablanca.
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Coronavirus is a virus that was unknown before the outbreak in Wuhan (China) in December 2019 but today it has become a major public health problem. Indeed, Covid-19 is an infectious and acute disease but it can also be very deadly especially with certain comorbidities such as hypertension, diabetes, coronary heart disease, cerebral infarction or even chronic bronchitis. The qRT-PCR is the reference method for the diagnosis of Covid-19. Nevertheless, it is a delicate technique whose result depends on several parameters such as the quality of the sample, the RNA extraction steps, the cDNA synthesis, the choice of the amplification kit, the choice of controls, the analysis and the validation of the results. Any anomalies in these steps may hinder the obtaining of relevant and reliable biological results. The objective of this paper is to verify the importance and analytical efficiency of the endogenous and exogenous controls used in two diagnostic tests for Sars-cov2 by qRT-PCR commonly used in the laboratory of medical analysis and biology of reproduction, Labomac Casablanca.