The Influence of Incubation Temperatures 25°C versus 36°C on Sperm Survival after Improvement
Chaymaa Kabir-Idrissi , Achraf Zakaria , Mustafa Zakaria , Sabri Yassir , Abdelghani Mrini , Bouchra El Khalfi , Abdelaziz Soukri , Modou Mamoune Mbaye* , Noureddine Louanjli
DOI : 10.46890/SL.2020.v02i06006
Abstract
Just like in women, the causes of infertility in men are many and varied. To remedy this, several couples resort to medically assisted procreation (PAM). However, the latter comes up against the problem of the sperm quality of the donor. For this, subnormal semen samples are treated with specific chemical agents before artificial insemination to improve the fertilizing power of the sperm. Because sperm health plays a decisive role in male fertility, survival is correlated with the fertilizing power of sperm. It is in this perspective that our study is part of our study consisting in following the survival of the sperm after improvement and incubation at 25 °C versus 36 °C in order to find the optimal temperature of incubation for an apparent increase. Poor results from in vitro fertilization (-20%). The results of the effect of incubating samples at 25 °C and 36 °C on the mobility and vitality of human spermatozoa which were followed in different incubation times (1,2, 3 and 4h) showed that ‘after 1 hour of incubation the mobility and vitality at 25 °C dropped considerably compared to that of the sample at 36°C. After 2 hours of incubation time, we note that the two samples drop drastically but still with the samples at 36°C which have kept the best mobility and vitality. This same phenomenon was also noted at 2 and 3 hours of incubation at 25 and 36 °C. It should be remembered that the most favorable temperature was 36°C because the highest fertilization rate was noted at this temperature.
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Just like in women, the causes of infertility in men are many and varied. To remedy this, several couples resort to medically assisted procreation (PAM). However, the latter comes up against the problem of the sperm quality of the donor. For this, subnormal semen samples are treated with specific chemical agents before artificial insemination to improve the fertilizing power of the sperm. Because sperm health plays a decisive role in male fertility, survival is correlated with the fertilizing power of sperm. It is in this perspective that our study is part of our study consisting in following the survival of the sperm after improvement and incubation at 25 °C versus 36 °C in order to find the optimal temperature of incubation for an apparent increase. Poor results from in vitro fertilization (-20%). The results of the effect of incubating samples at 25 °C and 36 °C on the mobility and vitality of human spermatozoa which were followed in different incubation times (1,2, 3 and 4h) showed that ‘after 1 hour of incubation the mobility and vitality at 25 °C dropped considerably compared to that of the sample at 36°C. After 2 hours of incubation time, we note that the two samples drop drastically but still with the samples at 36°C which have kept the best mobility and vitality. This same phenomenon was also noted at 2 and 3 hours of incubation at 25 and 36 °C. It should be remembered that the most favorable temperature was 36°C because the highest fertilization rate was noted at this temperature.